Hotspot

trnN-GUU-trnR-ACG

SSC · 39823-39979 · 157 bp

Back to Mammillaria

Pi 0.0338
MarkerSeek score 67.50
Primer available yes
Label trnN-GUU-trnR-ACG

Feature Metadata

label_name
trnN-GUU-trnR-ACG
feature_type
igs
region
SSC
length_bp
157
variable_sites
5
pi
0.0338
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-trnR-ACG_p1 ATTCGAACCTACGACCCATC TCAGGGGAGAATAGAGCACG 50.0 55.0 57.1 58.6 273-282 bp 1.0 72.2
trnN-GUU-trnR-ACG_p2 GATTCGAACCTACGACCCAT TCAGGGGAGAATAGAGCACG 50.0 55.0 57.1 58.6 274-283 bp 1.0 72.2
trnN-GUU-trnR-ACG_p3 AGGATTCGAACCTACGACCC TCAGGGGAGAATAGAGCACG 55.0 55.0 58.9 58.6 276-285 bp 1.0 72.1
trnN-GUU-trnR-ACG_p4 CTACCACTGAGCTACTGAGGA TCAGGGGAGAATAGAGCACG 52.4 55.0 58.0 58.6 235-244 bp 1.0 71.9
trnN-GUU-trnR-ACG_p5 TCTACCACTGAGCTACTGAGG TCAGGGGAGAATAGAGCACG 52.4 55.0 58.0 58.6 236-245 bp 1.0 71.8