Hotspot

trnL-UAG-rpl32

SSC · 37203-38204 · 1002 bp

Back to Mammillaria

Pi 0.00798
MarkerSeek score 63.30
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
1002
variable_sites
8
pi
0.00798
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 CTCGCACTGCTTCCTAAGAG GGGCAGCGTTAAAAGCTTTT 55.0 45.0 58.1 57.9 1171-1177 bp 1.0 78.7
trnL-UAG-rpl32_p2 CTCTCGCACTGCTTCCTAAG GGGCAGCGTTAAAAGCTTTT 55.0 45.0 58.1 57.9 1173-1179 bp 1.0 78.7
trnL-UAG-rpl32_p3 TCCTAAGAGCAGCGTGTCTA GGGCAGCGTTAAAAGCTTTT 50.0 45.0 57.9 57.9 1160-1166 bp 1.0 78.4
trnL-UAG-rpl32_p4 AGCAGCGTGTCTACCAATTT GGGCAGCGTTAAAAGCTTTT 45.0 45.0 57.8 57.9 1153-1159 bp 1.0 77.9
trnL-UAG-rpl32_p5 CTAAGAGCAGCGTGTCTACC GGGCAGCGTTAAAAGCTTTT 55.0 45.0 57.8 57.9 1158-1164 bp 1.0 77.8