Hotspot

rpl16-rps3

LSC · 11824-12852 · 1029 bp

Back to Mammillaria

Pi 0.00486

MarkerSeek score 61.60

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 1029
variable_sites: 5
pi: 0.00486
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	GTTCAAGTGCCTGAAGAGCA	TATTGCGCCTATACGGTTCG	50.0	50.0	58.4	57.9	1222 bp	1.0	76.7
rpl16-rps3_p2	GTTCAAGTGCCTGAAGAGCA	AAACCATTCGCGCGAAAATT	50.0	40.0	58.4	57.9	1245 bp	1.0	76.7
rpl16-rps3_p3	GTTCAAGTGCCTGAAGAGCA	TTCCCCTACAAACCATTCGC	50.0	50.0	58.4	58.2	1254 bp	1.0	75.8
rpl16-rps3_p4	GTTCAAGTGCCTGAAGAGCA	CCATTCGCGCGAAAATTGAT	50.0	45.0	58.4	58.2	1242 bp	1.0	75.8
rpl16-rps3_p5	AGGTTCAAGTGCCTGAAGAG	TATTGCGCCTATACGGTTCG	50.0	50.0	57.4	57.9	1224 bp	1.0	75.5