Hotspot

petB-petD

LSC · 6673-7652 · 980 bp

Back to Mammillaria

Pi 0.0051

MarkerSeek score 61.70

Primer available yes

Label petB-petD

Feature Metadata

label_name: petB-petD
feature_type: igs
region: LSC
length_bp: 980
variable_sites: 5
pi: 0.0051
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB-petD_p1	TGCCTCTTCTTACTGCCGTA	ACCCATCCCTTTTGCCAATT	50.0	45.0	58.4	58.0	1153-1157 bp	1.0	76.4
petB-petD_p2	CTTGCCTCTTCTTACTGCCG	ACCCATCCCTTTTGCCAATT	55.0	45.0	58.6	58.0	1155-1159 bp	1.0	74.9
petB-petD_p3	TCTTGCCTCTTCTTACTGCC	ACCCATCCCTTTTGCCAATT	50.0	45.0	57.2	58.0	1156-1160 bp	1.0	73.8
petB-petD_p4	TGCCTCTTCTTACTGCCGTA	CCCTTTTGCCAATTTAGCCC	50.0	50.0	58.4	57.6	1147-1151 bp	1.0	73.6
petB-petD_p5	TGCCTCTTCTTACTGCCGTA	GACCCATCCCTTTTGCCAAT	50.0	50.0	58.4	58.4	1154-1158 bp	1.0	73.3