primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	rps18	1	CCAGTTGAAGAACCCAAGCA	TGAAAACAAACCAGCAATCCA	20	21	50.0	38.1	58.3	56.8	0.0	0.0	2.489147	54	431	487-487	1.000000	60.9
rps18_p2	rps18	2	CCCCAGTTGAAGAACCCAAG	TGAAAACAAACCAGCAATCCA	20	21	55.0	38.1	58.4	56.8	0.0	0.0	2.559520	54	431	489-489	1.000000	60.3
rps18_p3	rps18	3	TGAAGAACCCAAGCAAGAGC	TGAAAACAAACCAGCAATCCA	20	21	50.0	38.1	58.4	56.8	0.0	0.0	2.571135	54	431	482-482	1.000000	60.3
rps18_p4	rps18	4	CCAGTTGAAGAACCCAAGCA	TTGAAAACAAACCAGCAATCCA	20	22	50.0	36.4	58.3	57.5	0.0	0.0	2.787009	54	431	488-488	1.000000	58.5
rps18_p5	rps18	5	CCCCAGTTGAAGAACCCAAG	TTGAAAACAAACCAGCAATCCA	20	22	55.0	36.4	58.4	57.5	0.0	0.0	2.857383	54	431	490-490	1.000000	58.0
petB-petD_p1	petB-petD	1	TGCCTCTTCTTACTGCCGTA	ACCCATCCCTTTTGCCAATT	20	20	50.0	45.0	58.4	58.0	0.0	0.0	0.490729	6673	7652	1153-1157	1.000000	76.4
petB-petD_p2	petB-petD	2	CTTGCCTCTTCTTACTGCCG	ACCCATCCCTTTTGCCAATT	20	20	55.0	45.0	58.6	58.0	0.0	0.0	0.679564	6673	7652	1155-1159	1.000000	74.9
petB-petD_p3	petB-petD	3	TCTTGCCTCTTCTTACTGCC	ACCCATCCCTTTTGCCAATT	20	20	50.0	45.0	57.2	58.0	0.0	0.0	0.813471	6673	7652	1156-1160	1.000000	73.8
petB-petD_p4	petB-petD	4	TGCCTCTTCTTACTGCCGTA	CCCTTTTGCCAATTTAGCCC	20	20	50.0	50.0	58.4	57.6	0.0	0.0	0.849005	6673	7652	1147-1151	1.000000	73.6
petB-petD_p5	petB-petD	5	TGCCTCTTCTTACTGCCGTA	GACCCATCCCTTTTGCCAAT	20	20	50.0	50.0	58.4	58.4	0.0	0.0	0.875467	6673	7652	1154-1158	1.000000	73.3
rpoA-rps11_p1	rpoA-rps11	1	TTCAACACACTTCCACTGCA	CGGAAGGGATGGAGCATTAC	20	20	45.0	55.0	57.9	58.1	0.0	0.0	0.237056	9324	9394	246-246	1.000000	79.1
rpoA-rps11_p2	rpoA-rps11	2	TTCAACACACTTCCACTGCA	GAAGGGATGGAGCATTACGG	20	20	45.0	55.0	57.9	58.1	0.0	0.0	0.237056	9324	9394	244-244	1.000000	79.1
rpoA-rps11_p3	rpoA-rps11	3	TTCAACACACTTCCACTGCA	GGAAGGGATGGAGCATTACG	20	20	45.0	55.0	57.9	58.1	0.0	0.0	0.237056	9324	9394	245-245	1.000000	79.1
rpoA-rps11_p4	rpoA-rps11	4	GGAGAGAGAATAAAGCGCCC	GAAGGGATGGAGCATTACGG	20	20	55.0	55.0	58.1	58.1	2.3	0.0	0.243377	9324	9394	294-294	1.000000	78.9
rpoA-rps11_p5	rpoA-rps11	5	GGAGAGAGAATAAAGCGCCC	GGAAGGGATGGAGCATTACG	20	20	55.0	55.0	58.1	58.1	2.3	0.0	0.243377	9324	9394	295-295	1.000000	78.9
rpl16-rps3_p1	rpl16-rps3	1	GTTCAAGTGCCTGAAGAGCA	TATTGCGCCTATACGGTTCG	20	20	50.0	50.0	58.4	57.9	0.0	4.2	0.456945	11824	12852	1222-1222	1.000000	76.7
rpl16-rps3_p2	rpl16-rps3	2	GTTCAAGTGCCTGAAGAGCA	AAACCATTCGCGCGAAAATT	20	20	50.0	40.0	58.4	57.9	0.0	43.9	0.461172	11824	12852	1245-1245	1.000000	76.7
rpl16-rps3_p3	rpl16-rps3	3	GTTCAAGTGCCTGAAGAGCA	TTCCCCTACAAACCATTCGC	20	20	50.0	50.0	58.4	58.2	0.0	0.0	0.573279	11824	12852	1254-1254	1.000000	75.8
rpl16-rps3_p4	rpl16-rps3	4	GTTCAAGTGCCTGAAGAGCA	CCATTCGCGCGAAAATTGAT	20	20	50.0	45.0	58.4	58.2	0.0	43.9	0.575931	11824	12852	1242-1242	1.000000	75.8
rpl16-rps3_p5	rpl16-rps3	5	AGGTTCAAGTGCCTGAAGAG	TATTGCGCCTATACGGTTCG	20	20	50.0	50.0	57.4	57.9	0.0	4.2	0.612607	11824	12852	1224-1224	1.000000	75.5
psaC_p1	psaC	1	TCGTCTGGTATTTGAATATGTGGT	GGTACGGGTTTTTGTCGGTA	24	20	37.5	50.0	58.0	57.8	0.0	0.0	4.177595	35212	35457	411-420	1.000000	48.5
psaC_p2	psaC	2	CTGGTAAATCATTGATTCGTCTGG	GGTACGGGTTTTTGTCGGTA	24	20	41.7	50.0	57.9	57.8	12.2	0.0	4.215159	35212	35457	427-436	1.000000	48.1
psaC_p3	psaC	3	TGGTAAATCATTGATTCGTCTGG	GGTACGGGTTTTTGTCGGTA	23	20	39.1	50.0	56.9	57.8	12.2	0.0	4.231642	35212	35457	426-435	1.000000	48.0
psaC_p4	psaC	4	GGTAAATCATTGATTCGTCTGGT	GGTACGGGTTTTTGTCGGTA	23	20	39.1	50.0	56.9	57.8	12.2	0.0	4.233473	35212	35457	425-434	1.000000	48.0
psaC_p5	psaC	5	CGTCTGGTATTTGAATATGTGGT	GGTACGGGTTTTTGTCGGTA	23	20	39.1	50.0	56.7	57.8	0.0	0.0	4.484991	35212	35457	410-419	1.000000	46.1
psaC-ndhD_p1	psaC-ndhD	1	TTTAGGGCCTGAAACAACCC	AGAACCTGCAAATATGGGCA	20	20	50.0	45.0	58.0	57.5	18.5	2.0	0.534397	35458	35609	242-252	1.000000	83.8
psaC-ndhD_p2	psaC-ndhD	2	CCGGAGTATGGGTCTAGCTT	AGAACCTGCAAATATGGGCA	20	20	55.0	45.0	58.3	57.5	0.0	2.0	0.824587	35458	35609	224-234	1.000000	82.1
psaC-ndhD_p3	psaC-ndhD	3	TGTGAATCTGCCTGTCCAAC	AGAACCTGCAAATATGGGCA	20	20	50.0	45.0	58.1	57.5	0.0	2.0	0.634240	35458	35609	286-296	1.000000	81.8
psaC-ndhD_p4	psaC-ndhD	4	TGCCTGTCCAACCGATTTTT	AGAACCTGCAAATATGGGCA	20	20	45.0	45.0	58.3	57.5	0.0	2.0	0.831954	35458	35609	278-288	1.000000	80.4
psaC-ndhD_p5	psaC-ndhD	5	CAGAGGACTGTGTTGGTTGT	AGAACCTGCAAATATGGGCA	20	20	50.0	45.0	57.7	57.5	0.0	2.0	0.862523	35458	35609	312-322	1.000000	79.4
trnL-UAG-rpl32_p1	trnL-UAG-rpl32	1	CTCGCACTGCTTCCTAAGAG	GGGCAGCGTTAAAAGCTTTT	20	20	55.0	45.0	58.1	57.9	0.0	26.5	0.228997	37203	38204	1171-1177	1.000000	78.7
trnL-UAG-rpl32_p2	trnL-UAG-rpl32	2	CTCTCGCACTGCTTCCTAAG	GGGCAGCGTTAAAAGCTTTT	20	20	55.0	45.0	58.1	57.9	0.0	26.5	0.228997	37203	38204	1173-1179	1.000000	78.7
trnL-UAG-rpl32_p3	trnL-UAG-rpl32	3	TCCTAAGAGCAGCGTGTCTA	GGGCAGCGTTAAAAGCTTTT	20	20	50.0	45.0	57.9	57.9	0.0	26.5	0.273193	37203	38204	1160-1166	1.000000	78.4
trnL-UAG-rpl32_p4	trnL-UAG-rpl32	4	AGCAGCGTGTCTACCAATTT	GGGCAGCGTTAAAAGCTTTT	20	20	45.0	45.0	57.8	57.9	0.0	26.5	0.333920	37203	38204	1153-1159	1.000000	77.9
trnL-UAG-rpl32_p5	trnL-UAG-rpl32	5	CTAAGAGCAGCGTGTCTACC	GGGCAGCGTTAAAAGCTTTT	20	20	55.0	45.0	57.8	57.9	0.0	26.5	0.345211	37203	38204	1158-1164	1.000000	77.8
trnN-GUU-trnR-ACG_p1	trnN-GUU-trnR-ACG	1	ATTCGAACCTACGACCCATC	TCAGGGGAGAATAGAGCACG	20	20	50.0	55.0	57.1	58.6	0.0	0.0	1.481079	39823	39979	273-282	1.000000	72.2
trnN-GUU-trnR-ACG_p2	trnN-GUU-trnR-ACG	2	GATTCGAACCTACGACCCAT	TCAGGGGAGAATAGAGCACG	20	20	50.0	55.0	57.1	58.6	0.0	0.0	1.481079	39823	39979	274-283	1.000000	72.2
trnN-GUU-trnR-ACG_p3	trnN-GUU-trnR-ACG	3	AGGATTCGAACCTACGACCC	TCAGGGGAGAATAGAGCACG	20	20	55.0	55.0	58.9	58.6	15.0	0.0	1.486577	39823	39979	276-285	1.000000	72.1
trnN-GUU-trnR-ACG_p4	trnN-GUU-trnR-ACG	4	CTACCACTGAGCTACTGAGGA	TCAGGGGAGAATAGAGCACG	21	20	52.4	55.0	58.0	58.6	11.2	0.0	1.602297	39823	39979	235-244	1.000000	71.9
trnN-GUU-trnR-ACG_p5	trnN-GUU-trnR-ACG	5	TCTACCACTGAGCTACTGAGG	TCAGGGGAGAATAGAGCACG	21	20	52.4	55.0	58.0	58.6	0.0	0.0	1.602297	39823	39979	236-245	1.000000	71.8
rrn5-rrn4.5_p1	rrn5-rrn4.5	1	AGTATCGTCACCGCAGTAGA	CAGCTGAGGCATCCTAACAG	20	20	50.0	55.0	58.0	58.0	0.0	8.4	0.083276	40375	41154	855-897	1.000000	91.3
rrn5-rrn4.5_p2	rrn5-rrn4.5	2	TATCGTCACCGCAGTAGAGT	CAGCTGAGGCATCCTAACAG	20	20	50.0	55.0	58.0	58.0	0.0	8.4	0.083276	40375	41154	853-895	1.000000	91.3
rrn5-rrn4.5_p3	rrn5-rrn4.5	3	AGTATCGTCACCGCAGTAGA	GCTGAGGCATCCTAACAGAC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.087359	40375	41154	853-895	1.000000	91.3
rrn5-rrn4.5_p4	rrn5-rrn4.5	4	TATCGTCACCGCAGTAGAGT	GCTGAGGCATCCTAACAGAC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.087359	40375	41154	851-893	1.000000	91.3
rrn5-rrn4.5_p5	rrn5-rrn4.5	5	AGTTCCTCTACGCCTAGGAC	CAGCTGAGGCATCCTAACAG	20	20	55.0	55.0	57.9	58.0	14.8	8.4	0.104856	40375	41154	803-845	1.000000	91.2
trnI-GAU_p1	trnI-GAU	1	AATCGCGGCGTTCTTCTATT	GGGAAGGATGAATCGCTCTC	20	20	45.0	55.0	58.1	57.8	22.9	0.0	0.226387	44505	44539	160-160	1.000000	79.7
trnI-GAU_p2	trnI-GAU	2	AATCGCGGCGTTCTTCTATT	GGAAGGATGAATCGCTCTCG	20	20	45.0	55.0	58.1	58.2	22.9	0.0	0.286967	44505	44539	159-159	1.000000	79.2
trnI-GAU_p3	trnI-GAU	3	GTAATCGCGGCGTTCTTCTA	GGGAAGGATGAATCGCTCTC	20	20	50.0	55.0	58.2	57.8	22.9	0.0	0.330977	44505	44539	162-162	1.000000	78.8
trnI-GAU_p4	trnI-GAU	4	AATCGCGGCGTTCTTCTATT	AACGGAGAGTTGTATGCTGC	20	20	45.0	50.0	58.1	58.3	22.9	0.0	0.337214	44505	44539	184-184	1.000000	78.6
trnI-GAU_p5	trnI-GAU	5	AATCGCGGCGTTCTTCTATT	GGAGAGTTGTATGCTGCGTT	20	20	45.0	50.0	58.1	58.3	22.9	0.0	0.337214	44505	44539	181-181	1.000000	78.6
trnV-GAC-rps12_p1	trnV-GAC-rps12	1	GTCAAGGTGACACTCTACCG	TGGCAACTTTACGTAAGGCA	20	20	55.0	45.0	57.7	57.7	5.4	16.5	0.606988	47590	48337	829-829	1.000000	75.4
trnV-GAC-rps12_p2	trnV-GAC-rps12	2	GGTGGGGAGCAAGTCAAAAT	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	58.4	57.7	0.0	16.5	0.625102	47590	48337	906-906	1.000000	75.3
trnV-GAC-rps12_p3	trnV-GAC-rps12	3	CAGGCTCGAACTGATGACTT	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.6	57.7	10.4	16.5	0.634499	47590	48337	856-856	1.000000	75.2
trnV-GAC-rps12_p4	trnV-GAC-rps12	4	TGACACTCTACCGCTGAGTT	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	58.4	57.7	0.0	16.5	0.640368	47590	48337	822-822	1.000000	75.2
trnV-GAC-rps12_p5	trnV-GAC-rps12	5	TCAGGCTCGAACTGATGACT	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	58.4	57.7	13.7	16.5	0.707364	47590	48337	857-857	1.000000	74.6
ndhB-ycf2_p1	ndhB-ycf2	1	GGTTCGCTCGAGAAATTCCT	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.0	58.0	18.7	7.6	0.020741	50933	51210	878-985	1.000000	82.8
ndhB-ycf2_p2	ndhB-ycf2	2	CGGGAATTTCAATTGCGCTT	GTTGGGCGCTTTAACCATTC	20	20	45.0	50.0	58.0	58.0	9.7	7.6	0.014285	50933	51210	980-1087	1.000000	82.6
ndhB-ycf2_p3	ndhB-ycf2	3	GGTTCGCTCGAGAAATTCCT	CGATCAGACCAACCTTCTGG	20	20	50.0	55.0	58.0	58.0	18.7	0.0	0.027276	50933	51210	706-829	1.000000	81.7
ndhB-ycf2_p4	ndhB-ycf2	4	CGGGAATTTCAATTGCGCTT	CGATCAGACCAACCTTCTGG	20	20	45.0	55.0	58.0	58.0	9.7	0.0	0.020820	50933	51210	808-931	1.000000	81.5
ndhB-ycf2_p5	ndhB-ycf2	5	CCTGTTGTTCCGATGGAGAG	GTTGGGCGCTTTAACCATTC	20	20	55.0	50.0	58.0	58.0	0.0	7.6	0.024937	50933	51210	1443-1443	0.500000	59.8
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCCGCAG	20	20	45.0	45.0	58.0	57.9	1.4	0.0	0.171127	65135	65624	577-577	1.000000	79.2
atpH-atpI_p2	atpH-atpI	2	ATAACGGAAGCGGCAGAAAT	TACCTGTCATGTTCCTCGGA	20	20	45.0	50.0	58.0	57.8	1.4	0.0	0.263769	65135	65624	626-626	1.000000	78.4
atpH-atpI_p3	atpH-atpI	3	CAGGCCCAATAGAAGCAAGT	TTTTGCAACTTTAGCCGCAG	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.267529	65135	65624	618-618	1.000000	78.4
atpH-atpI_p4	atpH-atpI	4	AGCCAATCCAGCAGCAATAA	TTTTGCAACTTTAGCCGCAG	20	20	45.0	45.0	57.9	57.9	0.0	0.0	0.281186	65135	65624	593-593	1.000000	78.3
atpH-atpI_p5	atpH-atpI	5	GCAGCAGTACCTTGACCAAT	TTTTGCAACTTTAGCCGCAG	20	20	50.0	45.0	58.2	57.9	0.0	0.0	0.306355	65135	65624	640-640	1.000000	78.0