Hotspot

psbA

LSC · 550-1611 · 1062 bp

Back to Malvaviscus

Pi 0

MarkerSeek score 40.00

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	AGGAGCAATACCAACACTCT	TAATTTGTGCGCTTGGGAGT	45.0	45.0	56.1	58.1	1200 bp	1.0	64.4
psbA_p2	AAGGAGCAATACCAACACTCT	TAATTTGTGCGCTTGGGAGT	42.9	45.0	56.9	58.1	1201 bp	1.0	62.6
psbA_p3	AGGAGCAATACCAACACTCTT	TAATTTGTGCGCTTGGGAGT	42.9	45.0	56.9	58.1	1200 bp	1.0	62.6
psbA_p4	GGAGCAATACCAACACTCTTG	TAATTTGTGCGCTTGGGAGT	47.6	45.0	56.9	58.1	1199 bp	1.0	62.3
psbA_p5	AGGAGCAATACCAACACTCTTG	TAATTTGTGCGCTTGGGAGT	45.5	45.0	58.3	58.1	1200 bp	1.0	60.7