Hotspot

atpF

LSC · 12302-13669 · 1368 bp

Back to Malvaviscus

Pi 0

MarkerSeek score 40.00

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1368
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	CGTCGGCTCTAATAGTTACCA	TGGAAAAGGAGTCAAAGTGA	47.6	40.0	57.0	54.4	1547 bp	1.0	40.0
atpF_p2	TCGTCGGCTCTAATAGTTACC	TGGAAAAGGAGTCAAAGTGA	47.6	40.0	56.7	54.4	1548 bp	1.0	40.0
atpF_p3	CGTCGGCTCTAATAGTTACCA	ATGGAAAAGGAGTCAAAGTGA	47.6	38.1	57.0	55.0	1548 bp	1.0	40.0
atpF_p4	CGTCGGCTCTAATAGTTACCA	TGGAAAAGGAGTCAAAGTGAT	47.6	38.1	57.0	55.0	1547 bp	1.0	40.0
atpF_p5	TCGTCGGCTCTAATAGTTACCA	TGGAAAAGGAGTCAAAGTGA	45.5	40.0	58.5	54.4	1548 bp	1.0	40.0