Hotspot

trnT-GGU-psbD

LSC · 33523-35027 · 1505 bp

Back to Malvaviscus

Pi 0.000664
MarkerSeek score 59.10
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1505
variable_sites
1
pi
0.000664
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG GAAACGGTCCCTACGTAACC 55.0 55.0 58.1 58.0 1647 bp 1.0 79.5
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA GAAACGGTCCCTACGTAACC 50.0 55.0 58.1 58.0 1650 bp 1.0 79.3
trnT-GGU-psbD_p3 ACGGCCCTTTTAACTCAGTG GAAACGGTCCCTACGTAACC 50.0 55.0 58.1 58.0 1664 bp 1.0 79.2
trnT-GGU-psbD_p4 AATTGAATCGAACGGCCCTT GAAACGGTCCCTACGTAACC 45.0 55.0 58.2 58.0 1675 bp 1.0 78.7
trnT-GGU-psbD_p5 ATTGAATCGAACGGCCCTTT GAAACGGTCCCTACGTAACC 45.0 55.0 58.2 58.0 1674 bp 1.0 78.7