Hotspot

trnS-GCU-trnG-UCC

LSC · 8453-9323 · 871 bp

Back to Malvaviscus

Pi 0

MarkerSeek score 40.00

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 871
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	ATGAATCAAACCGAGGGACC	50.0	50.0	58.0	57.6	986 bp	1.0	76.6
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	ATGAATCAAACCGAGGGACC	50.0	50.0	58.0	57.6	985 bp	1.0	76.6
trnS-GCU-trnG-UCC_p3	CGCACAACGGATTAGCAATC	ATGAATCAAACCGAGGGACC	50.0	50.0	57.9	57.6	1001 bp	1.0	75.9
trnS-GCU-trnG-UCC_p4	ACGGAAAGAGAGGGATTCGA	ATGAATCAAACCGAGGGACC	50.0	50.0	58.2	57.6	1040 bp	1.0	75.4
trnS-GCU-trnG-UCC_p5	TCGCACAACGGATTAGCAAT	ATGAATCAAACCGAGGGACC	45.0	50.0	58.3	57.6	1002 bp	1.0	74.5