Hotspot

trnK-UUU-rps16

LSC · 4516-5408 · 893 bp

Back to Malvaviscus

Pi 0
MarkerSeek score 40.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
893
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AAAAGGAGCTCAACCCACAG 50.0 50.0 58.2 58.0 1021 bp 1.0 78.5
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT AAAAGGAGCTCAACCCACAG 50.0 50.0 58.2 58.0 1020 bp 1.0 78.5
trnK-UUU-rps16_p3 TACCGTTGAGTTAGCAACCC AAAAGGAGCTCAACCCACAG 50.0 50.0 57.5 58.0 1007 bp 1.0 76.2
trnK-UUU-rps16_p4 AAAGCCGAGTACTCTACCGT AAGAAGGCGGGGGTTTTTAC 50.0 50.0 58.2 58.4 981 bp 1.0 75.6
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT AAGAAGGCGGGGGTTTTTAC 50.0 50.0 58.2 58.4 980 bp 1.0 75.6