Hotspot

matK-trnK-UUU

LSC · 3763-4478 · 716 bp

Back to Malvaviscus

Pi 0

MarkerSeek score 40.00

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 716
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	TATAGGAAATCATGCCGGCG	TTGGGGGTTGCTAACTCAAC	50.0	50.0	57.9	58.0	816 bp	1.0	79.2
matK-trnK-UUU_p2	CATGCCGGCGAGATCTATTT	TTGGGGGTTGCTAACTCAAC	50.0	50.0	58.2	58.0	806 bp	1.0	78.3
matK-trnK-UUU_p3	TATAGGAAATCATGCCGGCG	AACGGTAGAGTACTCGGCTT	50.0	50.0	57.9	58.2	799 bp	1.0	77.9
matK-trnK-UUU_p4	TATAGGAAATCATGCCGGCG	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.9	58.2	798 bp	1.0	77.9
matK-trnK-UUU_p5	CATGCCGGCGAGATCTATTT	ACGGTAGAGTACTCGGCTTT	50.0	50.0	58.2	58.2	788 bp	1.0	77.1