primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.203648	90	549	529-529	1.000000	78.4
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.208010	90	549	543-543	1.000000	78.3
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	90	549	542-542	1.000000	78.3
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	90	549	544-544	1.000000	78.3
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	20	20	55.0	55.0	58.1	58.3	0.0	0.0	0.465225	90	549	578-578	1.000000	76.3
psbA_p1	psbA	1	AGGAGCAATACCAACACTCT	TAATTTGTGCGCTTGGGAGT	20	20	45.0	45.0	56.1	58.1	0.0	9.7	1.949579	550	1611	1200-1200	1.000000	64.4
psbA_p2	psbA	2	AAGGAGCAATACCAACACTCT	TAATTTGTGCGCTTGGGAGT	21	20	42.9	45.0	56.9	58.1	0.0	9.7	2.173769	550	1611	1201-1201	1.000000	62.6
psbA_p3	psbA	3	AGGAGCAATACCAACACTCTT	TAATTTGTGCGCTTGGGAGT	21	20	42.9	45.0	56.9	58.1	0.0	9.7	2.173769	550	1611	1200-1200	1.000000	62.6
psbA_p4	psbA	4	GGAGCAATACCAACACTCTTG	TAATTTGTGCGCTTGGGAGT	21	20	47.6	45.0	56.9	58.1	0.0	9.7	2.206459	550	1611	1199-1199	1.000000	62.3
psbA_p5	psbA	5	AGGAGCAATACCAACACTCTTG	TAATTTGTGCGCTTGGGAGT	22	20	45.5	45.0	58.3	58.1	0.0	9.7	2.416324	550	1611	1200-1200	1.000000	60.7
matK_p1	matK	1	CTCTTCATCATTTCGGATCGGA	AACCTACTTGTCTCCGAGGT	22	20	45.5	50.0	58.0	57.7	0.0	0.0	2.323382	2248	3762	1697-1697	1.000000	61.4
matK_p2	matK	2	TCTCTTCATCATTTCGGATCGG	AACCTACTTGTCTCCGAGGT	22	20	45.5	50.0	58.0	57.7	0.0	0.0	2.323382	2248	3762	1698-1698	1.000000	61.4
matK_p3	matK	3	TCTTCATCATTTCGGATCGGA	AACCTACTTGTCTCCGAGGT	21	20	42.9	50.0	56.9	57.7	0.0	0.0	2.401533	2248	3762	1696-1696	1.000000	60.8
matK_p4	matK	4	TCTTCATCATTTCGGATCGGAA	AACCTACTTGTCTCCGAGGT	22	20	40.9	50.0	57.6	57.7	11.4	0.0	2.706387	2248	3762	1696-1696	1.000000	58.3
matK_p5	matK	5	CTCTTCATCATTTCGGATCGG	AACCTACTTGTCTCCGAGGT	21	20	47.6	50.0	56.6	57.7	0.0	0.0	2.720387	2248	3762	1697-1697	1.000000	58.2
matK-trnK-UUU_p1	matK-trnK-UUU	1	TATAGGAAATCATGCCGGCG	TTGGGGGTTGCTAACTCAAC	20	20	50.0	50.0	57.9	58.0	23.9	2.1	0.103600	3763	4478	816-816	1.000000	79.2
matK-trnK-UUU_p2	matK-trnK-UUU	2	CATGCCGGCGAGATCTATTT	TTGGGGGTTGCTAACTCAAC	20	20	50.0	50.0	58.2	58.0	23.9	2.1	0.209488	3763	4478	806-806	1.000000	78.3
matK-trnK-UUU_p3	matK-trnK-UUU	3	TATAGGAAATCATGCCGGCG	AACGGTAGAGTACTCGGCTT	20	20	50.0	50.0	57.9	58.2	23.9	17.1	0.261726	3763	4478	799-799	1.000000	77.9
matK-trnK-UUU_p4	matK-trnK-UUU	4	TATAGGAAATCATGCCGGCG	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	57.9	58.2	23.9	17.1	0.261726	3763	4478	798-798	1.000000	77.9
matK-trnK-UUU_p5	matK-trnK-UUU	5	CATGCCGGCGAGATCTATTT	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	58.2	58.2	23.9	17.1	0.367614	3763	4478	788-788	1.000000	77.1
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	AAAAGGAGCTCAACCCACAG	20	20	50.0	50.0	58.2	58.0	20.3	17.2	0.181537	4516	5408	1021-1021	1.000000	78.5
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	AAAAGGAGCTCAACCCACAG	20	20	50.0	50.0	58.2	58.0	20.3	17.2	0.181537	4516	5408	1020-1020	1.000000	78.5
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TACCGTTGAGTTAGCAACCC	AAAAGGAGCTCAACCCACAG	20	20	50.0	50.0	57.5	58.0	0.0	17.2	0.473875	4516	5408	1007-1007	1.000000	76.2
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAAGCCGAGTACTCTACCGT	AAGAAGGCGGGGGTTTTTAC	20	20	50.0	50.0	58.2	58.4	20.3	0.0	0.544084	4516	5408	981-981	1.000000	75.6
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	AAGAAGGCGGGGGTTTTTAC	20	20	50.0	50.0	58.2	58.4	20.3	0.0	0.544084	4516	5408	980-980	1.000000	75.6
rps16_p1	rps16	1	TGAATCATCCACACCCTCTT	TGGATCGACTCAACCTATGAC	20	21	45.0	47.6	56.1	56.9	0.0	0.0	3.955512	5409	6536	1229-1229	1.000000	48.4
rps16_p2	rps16	2	TGAATCATCCACACCCTCTTT	TGGATCGACTCAACCTATGAC	21	21	42.9	47.6	56.9	56.9	0.0	0.0	4.174128	5409	6536	1229-1229	1.000000	46.6
rps16_p3	rps16	3	ATGAATCATCCACACCCTCT	TGGATCGACTCAACCTATGAC	20	21	45.0	47.6	55.9	56.9	0.0	0.0	4.193923	5409	6536	1230-1230	1.000000	46.4
rps16_p4	rps16	4	TGAATCATCCACACCCTCTT	GGATCGACTCAACCTATGACT	20	21	45.0	47.6	56.1	56.6	0.0	0.0	4.241224	5409	6536	1228-1228	1.000000	46.1
rps16_p5	rps16	5	TGAATCATCCACACCCTCTT	TGGATCGACTCAACCTATGACT	20	22	45.0	45.5	56.1	58.4	0.0	0.0	4.245654	5409	6536	1229-1229	1.000000	46.0
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CAATCCGACGCTTTAGTCCA	ATGAATCAAACCGAGGGACC	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.424641	8453	9323	986-986	1.000000	76.6
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AATCCGACGCTTTAGTCCAC	ATGAATCAAACCGAGGGACC	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.426029	8453	9323	985-985	1.000000	76.6
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	CGCACAACGGATTAGCAATC	ATGAATCAAACCGAGGGACC	20	20	50.0	50.0	57.9	57.6	0.0	0.0	0.511210	8453	9323	1001-1001	1.000000	75.9
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	ACGGAAAGAGAGGGATTCGA	ATGAATCAAACCGAGGGACC	20	20	50.0	50.0	58.2	57.6	0.0	0.0	0.571407	8453	9323	1040-1040	1.000000	75.4
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	TCGCACAACGGATTAGCAAT	ATGAATCAAACCGAGGGACC	20	20	45.0	50.0	58.3	57.6	0.0	0.0	0.687118	8453	9323	1002-1002	1.000000	74.5
trnG-UCC_p1	trnG-UCC	1	CCAATTCCCCTGTTCGACAA	TGGAAAAACTAAAGAACATGCGA	20	23	50.0	34.8	58.1	57.2	0.0	0.0	3.855219	9324	10239	1087-1087	1.000000	49.2
trnG-UCC_p2	trnG-UCC	2	ATTCCCCTGTTCGACAAAGG	TGGAAAAACTAAAGAACATGCGA	20	23	50.0	34.8	57.8	57.2	0.0	0.0	3.959879	9324	10239	1084-1084	1.000000	48.3
trnG-UCC_p3	trnG-UCC	3	TTGATTACGCCCAATTCCCC	TGGAAAAACTAAAGAACATGCGA	20	23	50.0	34.8	58.2	57.2	0.0	0.0	3.989212	9324	10239	1097-1097	1.000000	48.1
trnG-UCC_p4	trnG-UCC	4	CCAATTCCCCTGTTCGACAA	TGCTAATGCATCGTTGAATT	20	20	50.0	35.0	58.1	54.0	0.0	0.0	4.133096	9324	10239	1022-1022	1.000000	46.9
trnG-UCC_p5	trnG-UCC	5	CCAATTCCCCTGTTCGACAA	TGGAAAAACTAAAGAACATGCGAA	20	24	50.0	33.3	58.1	57.9	0.0	0.0	4.233507	9324	10239	1087-1087	1.000000	46.1
atpA_p1	atpA	1	AGCCTTCCAAGCTAACGATG	GCCCGCTTACGAAAAGTAGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.030268	10709	12232	2415-2415	1.000000	79.8
atpA_p2	atpA	2	CCTAGCCTTCCAAGCTAACG	GCCCGCTTACGAAAAGTAGA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.059104	10709	12232	2418-2418	1.000000	79.5
atpA_p3	atpA	3	AGCCTTCCAAGCTAACGATG	AATGGAGGCGGATCAGTTTC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.073961	10709	12232	2395-2395	1.000000	79.4
atpA_p4	atpA	4	AGCCTTCCAAGCTAACGATG	GAAATGGAGGCGGATCAGTT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.073961	10709	12232	2397-2397	1.000000	79.4
atpA_p5	atpA	5	CCTAGCCTTCCAAGCTAACG	AATGGAGGCGGATCAGTTTC	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.102798	10709	12232	2398-2398	1.000000	79.2
atpF_p1	atpF	1	CGTCGGCTCTAATAGTTACCA	TGGAAAAGGAGTCAAAGTGA	21	20	47.6	40.0	57.0	54.4	0.0	0.0	5.571360	12302	13669	1547-1547	1.000000	40.0
atpF_p2	atpF	2	TCGTCGGCTCTAATAGTTACC	TGGAAAAGGAGTCAAAGTGA	21	20	47.6	40.0	56.7	54.4	0.0	0.0	5.845423	12302	13669	1548-1548	1.000000	40.0
atpF_p3	atpF	3	CGTCGGCTCTAATAGTTACCA	ATGGAAAAGGAGTCAAAGTGA	21	21	47.6	38.1	57.0	55.0	0.0	0.0	5.943013	12302	13669	1548-1548	1.000000	40.0
atpF_p4	atpF	4	CGTCGGCTCTAATAGTTACCA	TGGAAAAGGAGTCAAAGTGAT	21	21	47.6	38.1	57.0	55.0	0.0	0.0	5.943013	12302	13669	1547-1547	1.000000	40.0
atpF_p5	atpF	5	TCGTCGGCTCTAATAGTTACCA	TGGAAAAGGAGTCAAAGTGA	22	20	45.5	40.0	58.5	54.4	0.0	0.0	6.049883	12302	13669	1548-1548	1.000000	40.0
atpH-atpI_p1	atpH-atpI	1	AATAGAAGCAAGCCCGACAG	GCGAATCCATGGAAGGTCAT	20	20	50.0	50.0	58.0	58.0	0.0	20.7	0.056966	14502	15664	1255-1263	1.000000	79.5
atpH-atpI_p2	atpH-atpI	2	ATAACGGAAGCGGCAGAAAT	GCGAATCCATGGAAGGTCAT	20	20	45.0	50.0	58.0	58.0	1.4	20.7	0.065515	14502	15664	1221-1229	1.000000	79.5
atpH-atpI_p3	atpH-atpI	3	TACCTTGACCAACTCCAGGT	GCGAATCCATGGAAGGTCAT	20	20	50.0	50.0	57.9	58.0	0.0	20.7	0.139407	14502	15664	1277-1285	1.000000	78.9
atpH-atpI_p4	atpH-atpI	4	AATAGAAGCAAGCCCGACAG	TTTTGCAACTTTAGCTGCGG	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.162578	14502	15664	1284-1292	1.000000	78.7
atpH-atpI_p5	atpH-atpI	5	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	20	20	45.0	45.0	58.0	57.9	1.4	0.0	0.171127	14502	15664	1250-1258	1.000000	78.6
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	GAAACGGTCCCTACGTAACC	20	20	55.0	55.0	58.1	58.0	7.6	5.0	0.067776	33523	35027	1647-1647	1.000000	79.5
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	GAAACGGTCCCTACGTAACC	20	20	50.0	55.0	58.1	58.0	0.0	5.0	0.089930	33523	35027	1650-1650	1.000000	79.3
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	ACGGCCCTTTTAACTCAGTG	GAAACGGTCCCTACGTAACC	20	20	50.0	55.0	58.1	58.0	0.0	5.0	0.111499	33523	35027	1664-1664	1.000000	79.2
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	AATTGAATCGAACGGCCCTT	GAAACGGTCCCTACGTAACC	20	20	45.0	55.0	58.2	58.0	0.0	5.0	0.169520	33523	35027	1675-1675	1.000000	78.7
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	ATTGAATCGAACGGCCCTTT	GAAACGGTCCCTACGTAACC	20	20	45.0	55.0	58.2	58.0	0.0	5.0	0.169520	33523	35027	1674-1674	1.000000	78.7