Hotspot

rpl16

LSC · 84152-85685 · 1534 bp

Back to Malva

Pi 0.00509
MarkerSeek score 59.10
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1534
variable_sites
16
pi
0.00509
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 TCCCAAGACAAGACTTCTTTCC AAAACGAACAGTTCTAAATTCT 45.5 27.3 57.9 52.3 1627-1659 bp 1.0 42.7
rpl16_p2 CCCAAGACAAGACTTCTTTCCT AAAACGAACAGTTCTAAATTCT 45.5 27.3 57.9 52.3 1626-1658 bp 1.0 42.7
rpl16_p3 TCCCAAGACAAGACTTCTTTCC AAAAACGAACAGTTCTAAATTCT 45.5 26.1 57.9 53.1 1628-1660 bp 1.0 42.7
rpl16_p4 TCCCAAGACAAGACTTCTTTCC ACGAACAGTTCTAAATTCTATAAGGA 45.5 30.8 57.9 56.2 1624-1654 bp 0.8 33.9
rpl16_p5 CCCAAGACAAGACTTCTTTCCT ACGAACAGTTCTAAATTCTATAAGGA 45.5 30.8 57.9 56.2 1623-1653 bp 0.8 33.9