Hotspot

ndhD-psaC

SSC · 121142-121244 · 103 bp

Back to Malva

Pi 0.0427
MarkerSeek score 79.90
Primer available yes
Label ndhD-psaC

Feature Metadata

label_name
ndhD-psaC
feature_type
igs
region
SSC
length_bp
103
variable_sites
8
pi
0.0427
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD-psaC_p1 AAGAACCCGCAGATATTGGT AAGAGATGTGAATCCGCCTG 45.0 50.0 56.9 57.7 279-281 bp 1.0 71.7
ndhD-psaC_p2 AAGAACCCGCAGATATTGGT GAGATGTGAATCCGCCTGTC 45.0 55.0 56.9 58.4 277-279 bp 1.0 71.0
ndhD-psaC_p3 AAGAACCCGCAGATATTGGT GCCTGTCCAACGGATTTCTT 45.0 50.0 56.9 58.5 264-266 bp 1.0 70.8
ndhD-psaC_p4 AAGAACCCGCAGATATTGGT TCCAACGGATTTCTTGAGCG 45.0 50.0 56.9 58.6 259-261 bp 1.0 70.1
ndhD-psaC_p5 AAAGAACCCGCAGATATTGGT AAGAGATGTGAATCCGCCTG 42.9 50.0 57.6 57.7 280-282 bp 1.0 69.6