Hotspot

ndhF-rpl32

SSC · 115639-116810 · 1172 bp

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Pi 0.0172
MarkerSeek score 65.30
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1172
variable_sites
42
pi
0.0172
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGTTGCCGTCGGAAAAAGTA TGAAAAAGCTTTCAACGCCG 45.0 45.0 58.0 58.2 1364-1380 bp 1.0 81.4
ndhF-rpl32_p2 GGAAGTGGCACGAAAGGTAT TGAAAAAGCTTTCAACGCCG 50.0 45.0 57.9 58.2 1315-1331 bp 1.0 81.1
ndhF-rpl32_p3 TGTTGCCGTCGGAAAAAGTA GCCGCCCAATATCCTTTCTT 45.0 50.0 58.0 58.3 1348-1364 bp 1.0 80.3
ndhF-rpl32_p4 TTTGTTGCCGTCGGAAAAAG TGAAAAAGCTTTCAACGCCG 45.0 45.0 57.8 58.2 1366-1382 bp 1.0 80.2
ndhF-rpl32_p5 GGAAGTGGCACGAAAGGTAT GCCGCCCAATATCCTTTCTT 50.0 50.0 57.9 58.3 1299-1315 bp 1.0 80.0