Hotspot

accD-psaI

LSC · 61238-61855 · 618 bp

Back to Malva

Pi 0.0158
MarkerSeek score 65.70
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
618
variable_sites
17
pi
0.0158
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 GAGTTAGTTCAGCTCCACGG CCATTGCAATTGCCGGAAAT 55.0 45.0 58.0 58.0 718-745 bp 1.0 84.6
accD-psaI_p2 GAGTTAGTTCAGCTCCACGG GAGGGAAAGCTGCTGAAAGT 55.0 50.0 58.0 58.1 671-698 bp 1.0 84.3
accD-psaI_p3 AGTTCAGCTCCACGGTTTTT CCATTGCAATTGCCGGAAAT 45.0 45.0 57.9 58.0 713-740 bp 1.0 84.2
accD-psaI_p4 AGTTCAGCTCCACGGTTTTT GAGGGAAAGCTGCTGAAAGT 45.0 50.0 57.9 58.1 666-693 bp 1.0 84.0
accD-psaI_p5 GAGTTAGTTCAGCTCCACGG AAGAAGCCATTGCAATTGCC 55.0 45.0 58.0 57.9 724-751 bp 1.0 83.9