Hotspot

atpB-rbcL

LSC · 56646-57556 · 911 bp

Back to Malva

Pi 0.00839
MarkerSeek score 50.70
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
911
variable_sites
13
pi
0.00839
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 GCCGACTGGAACACTTAGAG GGTAGCATCGCCCTTTGTAA 55.0 50.0 58.0 58.0 1490-1499 bp 1.0 81.2
atpB-rbcL_p2 GCCGACTGGAACACTTAGAG TTCGCAGATCCTCTAGACGT 55.0 50.0 58.0 58.0 1652-1661 bp 1.0 81.0
atpB-rbcL_p3 GCCGACTGGAACACTTAGAG AACTCCGGGTTGAGGACTTA 55.0 50.0 58.0 58.0 1385-1387 bp 0.6 65.0
atpB-rbcL_p4 CGGTTCATTCATCTGACCGT AACTCCGGGTTGAGGACTTA 50.0 50.0 58.0 58.0 1802-1804 bp 0.6 64.8
atpB-rbcL_p5 AAAATTCGTCCTAGGGTCGC AACTCCGGGTTGAGGACTTA 50.0 50.0 58.0 58.0 1408-1410 bp 0.6 64.8