Hotspot

petN-psbM

LSC · 29360-30526 · 1167 bp

Back to Malva

Pi 0.00815
MarkerSeek score 65.30
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1167
variable_sites
20
pi
0.00815
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TCTTGCTTGGGCTGCTTTAA CATTTATTGCTACGGCGCTG 45.0 50.0 58.0 58.0 1325-1331 bp 1.0 81.5
petN-psbM_p2 TCTTGCTTGGGCTGCTTTAA CTACGGCGCTGTTCATTCTA 45.0 50.0 58.0 57.8 1316-1322 bp 1.0 80.0
petN-psbM_p3 TCTTGCTTGGGCTGCTTTAA TACGGCGCTGTTCATTCTAG 45.0 50.0 58.0 57.8 1315-1321 bp 1.0 80.0
petN-psbM_p4 AGTAAGTCTTGCTTGGGCTG CATTTATTGCTACGGCGCTG 50.0 50.0 57.8 58.0 1331-1337 bp 1.0 80.0
petN-psbM_p5 TCTTGCTTGGGCTGCTTTAA ATTTATTGCTACGGCGCTGT 45.0 45.0 58.0 58.3 1324-1330 bp 1.0 78.9