Hotspot

atpH-atpI

LSC · 13848-15065 · 1218 bp

Pi 0.00656

MarkerSeek score 66.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1218
variable_sites: 16
pi: 0.00656
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1292-1339 bp	1.0	81.6
atpH-atpI_p2	AATAGAAGCAAGCCCGACAG	TTTTGCAACTTTAGCTGCGG	50.0	45.0	58.0	57.9	1326-1373 bp	1.0	81.5
atpH-atpI_p3	AATAGAAGCAAGCCCGACAG	CGAATCCATGGAGGGTCATC	50.0	55.0	58.0	57.8	1296-1343 bp	1.0	81.3
atpH-atpI_p4	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	45.0	55.0	58.0	57.8	1262-1309 bp	1.0	81.3
atpH-atpI_p5	TACCTTGACCAACTCCAGGT	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.9	57.9	1348-1395 bp	1.0	80.8