Hotspot

trnR-UCU-atpA

LSC · 10275-10497 · 223 bp

Back to Malva

Pi 0.0197
MarkerSeek score 69.20
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
223
variable_sites
9
pi
0.0197
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.95
markerseek_score
69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 ACTCGTAATGAAAAGCGTCCA TCACTGAGGAAGCAGAAACG 42.9 50.0 58.0 57.8 374-398 bp 1.0 74.8
trnR-UCU-atpA_p2 GGTTCAAATCCTATTGGACGC TCACTGAGGAAGCAGAAACG 47.6 50.0 57.3 57.8 310-334 bp 1.0 70.4
trnR-UCU-atpA_p3 ACTCGTAATGAAAAGCGTCCA AAACGCTTTTGAAAGACGCT 42.9 40.0 58.0 57.2 359-383 bp 1.0 69.5
trnR-UCU-atpA_p4 CTCGTAATGAAAAGCGTCCA TCACTGAGGAAGCAGAAACG 45.0 50.0 56.2 57.8 373-397 bp 1.0 68.6
trnR-UCU-atpA_p5 ACTCGTAATGAAAAGCGTCC TCACTGAGGAAGCAGAAACG 45.0 50.0 56.2 57.8 374-398 bp 1.0 68.6