Hotspot

trnK-UUU-rps16

LSC · 4555-5363 · 809 bp

Back to Malva

Pi 0.00849
MarkerSeek score 67.90
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
809
variable_sites
15
pi
0.00849
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AAAAGGAGCTCAACCCACAG 50.0 50.0 58.2 58.0 917-976 bp 1.0 82.1
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT AAAAGGAGCTCAACCCACAG 50.0 50.0 58.2 58.0 916-975 bp 1.0 82.1
trnK-UUU-rps16_p3 TACCGTTGAGTTAGCAACCC AAAAGGAGCTCAACCCACAG 50.0 50.0 57.5 58.0 903-962 bp 1.0 79.8
trnK-UUU-rps16_p4 AAAGCCGAGTACTCTACCGT AAGAAGGCGGGGGTTTTTAC 50.0 50.0 58.2 58.4 877-936 bp 1.0 79.4
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT AAGAAGGCGGGGGTTTTTAC 50.0 50.0 58.2 58.4 876-935 bp 1.0 79.4