Hotspot

trnH-GUG-psbA

LSC · 76-564 · 489 bp

Back to Malva

Pi 0.0247
MarkerSeek score 64.10
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
489
variable_sites
23
pi
0.0247
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG TCACCGCTTTGGGTATTAGC 55.0 50.0 58.1 58.0 710-719 bp 1.0 84.1
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG CTGATGGTATGCCCCTAGGA 55.0 55.0 58.1 58.0 1058-1067 bp 1.0 82.3
trnH-GUG-psbA_p3 GATCCACTTGGCTACATCCG TCTGATGGTATGCCCCTAGG 55.0 55.0 58.1 58.0 1059-1068 bp 1.0 82.3
trnH-GUG-psbA_p4 CAATCCACTGCCTTGATCCA CTGATGGTATGCCCCTAGGA 50.0 55.0 57.9 58.0 1072-1081 bp 1.0 82.3
trnH-GUG-psbA_p5 CAATCCACTGCCTTGATCCA TCTGATGGTATGCCCCTAGG 50.0 55.0 57.9 58.0 1073-1082 bp 1.0 82.3