Hotspot

psbE-petL

LSC · 68396-69632 · 1237 bp

Back to Malus

Pi 0.00232
MarkerSeek score 53.00
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1237
variable_sites
33
pi
0.00232
unique_haplotype_count
20
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 AGAACGTTCTCCTGTGCTTC GTCGTATCTTGCTCAGACCG 50.0 55.0 57.8 58.2 1346-1395 bp 1.0 81.4
psbE-petL_p2 GTTCTCCTGTGCTTCCAGAC GTCGTATCTTGCTCAGACCG 55.0 55.0 58.2 58.2 1341-1390 bp 1.0 81.1
psbE-petL_p3 ACGAATAACCAACCCGCAAT GTCGTATCTTGCTCAGACCG 45.0 55.0 58.2 58.2 1432-1481 bp 1.0 81.0
psbE-petL_p4 TGCTGACGAATAACCAACCC GTCGTATCTTGCTCAGACCG 50.0 55.0 58.2 58.2 1437-1486 bp 1.0 80.9
psbE-petL_p5 AGAACGTTCTCCTGTGCTTC TCGTATCTTGCTCAGACCGA 50.0 50.0 57.8 58.3 1345-1394 bp 1.0 80.7