Hotspot

psaA-ycf3

LSC · 44899-45601 · 703 bp

Back to Malus

Pi 0.00183
MarkerSeek score 53.80
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
703
variable_sites
10
pi
0.00183
unique_haplotype_count
17
primer_available
yes
alignment_reliability
0.99
markerseek_score
53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 GCCCATTCCTCGAAAGAAGT TGGAAACAAGCAATAGCGCT 50.0 45.0 57.9 58.5 848-883 bp 1.0 79.2
psaA-ycf3_p2 GCCCATTCCTCGAAAGAAGT CAATAGCGCTTACTCCTGGT 50.0 50.0 57.9 57.4 838-873 bp 1.0 78.2
psaA-ycf3_p3 GCCCATTCCTCGAAAGAAGT GGAGGCTTGGTTCGATCAAG 50.0 55.0 57.9 58.6 879-914 bp 1.0 77.8
psaA-ycf3_p4 GCCCATTCCTCGAAAGAAGT GCAATAGCGCTTACTCCTGG 50.0 55.0 57.9 58.8 839-874 bp 1.0 76.8
psaA-ycf3_p5 TTGAGAAATGACCCGGTCTG TGGAAACAAGCAATAGCGCT 50.0 45.0 57.5 58.5 868-903 bp 1.0 76.3