Hotspot

trnT-GGU-psbD

LSC · 34219-35719 · 1501 bp

Back to Malus

Pi 0.0101
MarkerSeek score 68.50
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1501
variable_sites
115
pi
0.0101
unique_haplotype_count
32
primer_available
yes
alignment_reliability
0.98
markerseek_score
68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG GGCCAGACCAACCTACAAAA 55.0 50.0 58.1 58.0 1558-1895 bp 1.0 89.0
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA GGCCAGACCAACCTACAAAA 50.0 50.0 58.1 58.0 1561-1898 bp 1.0 88.8
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG TAGGCCAGACCAACCTACAA 55.0 50.0 58.1 58.0 1560-1897 bp 1.0 88.8
trnT-GGU-psbD_p4 GTGGTAGAGTAACGCCATGG ACCTACAAAAACGAAGCGGT 55.0 45.0 58.1 58.0 1548-1885 bp 1.0 88.7
trnT-GGU-psbD_p5 TCAGTGGTAGAGTAACGCCA TAGGCCAGACCAACCTACAA 50.0 50.0 58.1 58.0 1563-1900 bp 1.0 88.6