Hotspot

petN-psbM

LSC · 30304-31552 · 1249 bp

Back to Malus

Pi 0.00628
MarkerSeek score 59.20
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1249
variable_sites
65
pi
0.00628
unique_haplotype_count
22
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 AGTAAATCTCGCTTGGGCTG AAAACAGTCAGCCAAGGTGA 50.0 45.0 58.0 57.6 1313-1404 bp 1.0 83.5
petN-psbM_p2 AGTATGGGGAAGGAGTGGAC AAAACAGTCAGCCAAGGTGA 55.0 45.0 58.1 57.6 1256-1347 bp 1.0 83.1
petN-psbM_p3 GTATGGGGAAGGAGTGGACT AAAACAGTCAGCCAAGGTGA 55.0 45.0 58.1 57.6 1255-1346 bp 1.0 83.1
petN-psbM_p4 TATGGGGAAGGAGTGGACTC AAAACAGTCAGCCAAGGTGA 55.0 45.0 57.8 57.6 1254-1345 bp 1.0 82.6
petN-psbM_p5 GGGGAAGGAGTGGACTCTAG AAAACAGTCAGCCAAGGTGA 60.0 45.0 58.2 57.6 1251-1342 bp 1.0 82.3