Hotspot

rpoB-trnC-GCA

LSC · 27987-29218 · 1232 bp

Back to Malus

Pi 0.00347
MarkerSeek score 52.40
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1232
variable_sites
29
pi
0.00347
unique_haplotype_count
17
primer_available
yes
alignment_reliability
0.98
markerseek_score
52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 TAAGCCCTGCTCAATGAACC CCGGATTTGAACTGGGGAAA 50.0 50.0 57.9 58.1 1362-1404 bp 1.0 82.4
rpoB-trnC-GCA_p2 TAAGCCCTGCTCAATGAACC AAAAGGATTTGCAGTCCCCC 50.0 50.0 57.9 58.4 1344-1386 bp 1.0 80.3
rpoB-trnC-GCA_p3 TATTGCCTCATTTCCACCCC CCGGATTTGAACTGGGGAAA 50.0 50.0 57.5 58.1 1296-1338 bp 1.0 80.0
rpoB-trnC-GCA_p4 CTCTGTTAAGCCCTGCTCAA CCGGATTTGAACTGGGGAAA 50.0 50.0 57.5 58.1 1368-1410 bp 1.0 79.6
rpoB-trnC-GCA_p5 GTTCCTCTGTTAAGCCCTGC CCGGATTTGAACTGGGGAAA 55.0 50.0 58.5 58.1 1372-1414 bp 1.0 79.1