Hotspot

trnR-UCU-atpA

LSC · 10172-10960 · 789 bp

Back to Malus

Pi 0.0292
MarkerSeek score 70.30
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
789
variable_sites
170
pi
0.0292
unique_haplotype_count
33
primer_available
yes
alignment_reliability
0.98
markerseek_score
70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 GGTTCAAATCCTATTGGACGC AGACATTTACCGACGAAGCG 47.6 50.0 57.3 58.4 376-962 bp 1.0 75.5
trnR-UCU-atpA_p2 TGGATAGGACAGAGGTCTTCT AGACATTTACCGACGAAGCG 47.6 50.0 56.9 58.4 411-997 bp 1.0 72.6
trnR-UCU-atpA_p3 GGTTCAAATCCTATTGGACGC GGAAGCCCTTTTGAAAGAAGC 47.6 47.6 57.3 58.0 357-943 bp 1.0 70.2
trnR-UCU-atpA_p4 GTTCAAATCCTATTGGACGCA AGACATTTACCGACGAAGCG 42.9 50.0 56.6 58.4 375-961 bp 1.0 69.8
trnR-UCU-atpA_p5 GGTTCAAATCCTATTGGACGC ACCAAGACATTTACCGACGA 47.6 45.0 57.3 56.9 380-966 bp 1.0 69.6