Hotspot

rps16-trnQ-UUG

LSC · 6382-7193 · 812 bp

Back to Malus

Pi 0.00717
MarkerSeek score 65.00
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
812
variable_sites
35
pi
0.00717
unique_haplotype_count
28
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 872-902 bp 1.0 84.9
rps16-trnQ-UUG_p2 CCACAGCTGATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.6 58.1 882-912 bp 1.0 83.2
rps16-trnQ-UUG_p3 GCTTTCTGCCACATCGTTTT GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.6 58.1 849-879 bp 1.0 83.2
rps16-trnQ-UUG_p4 TCCACAGCTGATCATGTCCT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.4 58.1 883-913 bp 1.0 83.1
rps16-trnQ-UUG_p5 TCATGTCCTTCAAGTCGCAC GTGTCGACTAGAAATGGGGC 50.0 55.0 58.2 58.3 934-964 bp 1.0 82.5