Hotspot

trnH-GUG-psbA

LSC · 114-374 · 261 bp

Back to Malus

Pi 0.0124
MarkerSeek score 56.90
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
261
variable_sites
24
pi
0.0124
unique_haplotype_count
23
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 CAATCCACTGCCTTGATCCA GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 384-431 bp 1.0 86.4
trnH-GUG-psbA_p2 AATCCACTGCCTTGATCCAC GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 383-430 bp 1.0 86.4
trnH-GUG-psbA_p3 ATATTATGGGCGAACGACGG CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 381-428 bp 0.953488 83.8
trnH-GUG-psbA_p4 ATATTATGGGCGAACGACGG TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 384-431 bp 0.953488 83.7
trnH-GUG-psbA_p5 ATATTATGGGCGAACGACGG GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 431-478 bp 0.953488 83.3