Hotspot

rpl22-rps19

IRb · 89953-91133 · 1181 bp

Back to Mallotus

Pi 0.112

MarkerSeek score 65.10

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: IRb
length_bp: 1181
variable_sites: 55
pi: 0.112
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.39
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	TAGCACCTTCGTTGACTTCG	TCCCGGGCATCTACCATTAT	50.0	50.0	57.9	58.0	712-1635 bp	1.0	87.3
rpl22-rps19_p2	TAGCACCTTCGTTGACTTCG	AACAGTCGGACAAGTAGGGA	50.0	50.0	57.9	58.0	1153-2074 bp	1.0	86.5
rpl22-rps19_p3	TAGCACCTTCGTTGACTTCG	ACAGTCGGACAAGTAGGGAA	50.0	50.0	57.9	58.0	1152-2073 bp	1.0	86.5
rpl22-rps19_p4	GCAATTCCCTCAACACCAGA	AACAGTCGGACAAGTAGGGA	50.0	50.0	58.1	58.0	1542-2478 bp	1.0	85.6
rpl22-rps19_p5	GCAATTCCCTCAACACCAGA	ACAGTCGGACAAGTAGGGAA	50.0	50.0	58.1	58.0	1541-2477 bp	1.0	85.6