Hotspot

psbC-trnS-UGA

LSC · 39024-39220 · 197 bp

Back to Mallotus

Pi 0.0863

MarkerSeek score 80.80

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 197
variable_sites: 22
pi: 0.0863
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	GCAGCCGGATTTGAAAAAGG	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.3	58.2	276-328 bp	1.0	85.4
psbC-trnS-UGA_p2	CTGCAGCCGGATTTGAAAAA	GGGTTCGAATCCCTCTCTCT	45.0	55.0	57.6	58.2	278-330 bp	1.0	84.1
psbC-trnS-UGA_p3	TGCAGCCGGATTTGAAAAAG	GGGTTCGAATCCCTCTCTCT	45.0	55.0	57.6	58.2	277-329 bp	1.0	84.1
psbC-trnS-UGA_p4	TAGGTCATTTATGGCACGCG	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.4	58.2	314-366 bp	1.0	83.2
psbC-trnS-UGA_p5	GTAGGTCATTTATGGCACGC	GGGTTCGAATCCCTCTCTCT	50.0	55.0	57.0	58.2	315-367 bp	1.0	78.3