Hotspot

trnT-GGU-psbD

LSC · 34778-36592 · 1815 bp

Back to Mallotus

Pi 0.0586

MarkerSeek score 82.30

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1815
variable_sites: 135
pi: 0.0586
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GGCGTAAGTCATCGGTTCAA	CCCACAAAAACGAAACGGTC	50.0	50.0	58.3	58.2	1945-1949 bp	0.666667	68.8
trnT-GGU-psbD_p2	GGCGTAAGTCATCGGTTCAA	TCTCCGTAACCAGTCATCCA	50.0	50.0	58.3	57.8	1925-1929 bp	0.666667	68.4
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	TAATAGACCGGACCAACCCA	50.0	50.0	58.3	57.7	1961-1965 bp	0.666667	68.1
trnT-GGU-psbD_p4	GGCGTAAGTCATCGGTTCAA	AACCCACAAAAACGAAACGG	50.0	45.0	58.3	57.7	1947-1951 bp	0.666667	67.9
trnT-GGU-psbD_p5	GGCGTAAGTCATCGGTTCAA	GGTCTCTCCGTAACCAGTCA	50.0	55.0	58.3	58.5	1929-1933 bp	0.666667	66.5