Hotspot

trnD-GUC-trnY-GUA

LSC · 32347-32728 · 382 bp

Back to Mallotus

Pi 0.0532

MarkerSeek score 83.50

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 382
variable_sites: 30
pi: 0.0532
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	CGGTGCTCTGACCAATTGAA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.5	57.8	452-555 bp	1.0	82.3
trnD-GUC-trnY-GUA_p2	CGGTGCTCTGACCAATTGAA	GGCAATATGTCTACGCTGGT	50.0	50.0	58.5	57.8	451-554 bp	1.0	82.3
trnD-GUC-trnY-GUA_p3	CGGTGCTCTGACCAATTGAA	AATTGCTTCTTCCTGGGTCG	50.0	50.0	58.5	58.2	510-613 bp	1.0	81.9
trnD-GUC-trnY-GUA_p4	CGGTGCTCTGACCAATTGAA	TGGAATTGCTTCTTCCTGGG	50.0	50.0	58.5	57.8	513-616 bp	1.0	81.5
trnD-GUC-trnY-GUA_p5	CGGTGCTCTGACCAATTGAA	GGAATTGCTTCTTCCTGGGT	50.0	50.0	58.5	57.8	512-615 bp	1.0	81.5