Hotspot

psbM-trnD-GUC

LSC · 31351-32272 · 922 bp

Back to Mallotus

Pi 0.054

MarkerSeek score 82.50

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 922
variable_sites: 66
pi: 0.054
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	ACGCAAGAATATTTACTTCCA	TCCCCGGGATTGTAGTTCAA	33.3	50.0	53.2	58.3	979-1023 bp	1.0	49.3
psbM-trnD-GUC_p2	ACGCAAGAATATTTACTTCCA	TTCCCCGGGATTGTAGTTCA	33.3	50.0	53.2	58.3	980-1024 bp	1.0	49.3
psbM-trnD-GUC_p3	GTAGCAATAAACGCAAGAAT	TTCAATTGGTCAGAGCACCG	35.0	50.0	52.3	58.5	974-1018 bp	1.0	49.3
psbM-trnD-GUC_p4	GTAGCAATAAACGCAAGAAT	TCCCCGGGATTGTAGTTCAA	35.0	50.0	52.3	58.3	989-1033 bp	1.0	49.2
psbM-trnD-GUC_p5	GTAGCAATAAACGCAAGAAT	TTCCCCGGGATTGTAGTTCA	35.0	50.0	52.3	58.3	990-1034 bp	1.0	49.2