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petN-psbM

LSC · 30221-31245 · 1025 bp

Back to Mallotus

Pi 0.0847

MarkerSeek score 79.70

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1025
variable_sites: 92
pi: 0.0847
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ATTTGCTCCCAACAGAAGCA	TGCTACTGCCCTGTTCATTC	45.0	50.0	58.0	57.9	1132-1695 bp	1.0	88.7
petN-psbM_p2	ATTTGCTCCCAACAGAAGCA	CCCGACCCGTGTAATGAAAT	45.0	50.0	58.0	58.0	2113-2309 bp	0.666667	72.9
petN-psbM_p3	TTTCCCCAGTTCAAATCCGG	CCCGACCCGTGTAATGAAAT	50.0	50.0	58.1	58.0	2201-2397 bp	0.666667	72.1
petN-psbM_p4	GCCTGATCAACAAAAGACGC	CCCGACCCGTGTAATGAAAT	50.0	50.0	58.0	58.0	2176 bp	0.333333	52.8
petN-psbM_p5	ATTTGCTCCCAACAGAAGCA	CCCGGTAGCACATACACAAA	45.0	50.0	58.0	57.9	2072 bp	0.333333	52.5