Hotspot

atpF-atpH

LSC · 14395-14776 · 382 bp

Back to Mallotus

Pi 0.0444

MarkerSeek score 83.50

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 382
variable_sites: 21
pi: 0.0444
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CCAGTGACCCAAAGAAACGA	CGGAGGGAAAAATACGAGGT	50.0	50.0	58.0	57.3	520-558 bp	1.0	81.3
atpF-atpH_p2	CCAGTGACCCAAAGAAACGA	CGAGGCGGAGGGAAAAATAC	50.0	55.0	58.0	58.7	525-563 bp	1.0	81.2
atpF-atpH_p3	CCAGTGACCCAAAGAAACGA	GAGGCGGAGGGAAAAATACG	50.0	55.0	58.0	58.7	524-562 bp	1.0	81.2
atpF-atpH_p4	CCAGTGACCCAAAGAAACGA	GCGGAGGGAAAAATACGAGG	50.0	55.0	58.0	58.7	521-559 bp	1.0	81.2
atpF-atpH_p5	CCAGTGACCCAAAGAAACGA	GGCGGAGGGAAAAATACGAG	50.0	55.0	58.0	58.7	522-560 bp	1.0	81.2