Hotspot

atpA-atpF

LSC · 12998-13064 · 67 bp

Back to Mallotus

Pi 0.0601
MarkerSeek score 82.70
Primer available yes
Label atpA-atpF

Feature Metadata

label_name
atpA-atpF
feature_type
igs
region
LSC
length_bp
67
variable_sites
5
pi
0.0601
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.91
markerseek_score
82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpA-atpF_p1 CCGTCGCCTACTTGAAGTAC GCTTGACCGACGAGTTACAT 55.0 50.0 58.1 58.0 248-260 bp 1.0 84.8
atpA-atpF_p2 GTCGCCTACTTGAAGTACGG GCTTGACCGACGAGTTACAT 55.0 50.0 58.1 58.0 246-258 bp 1.0 84.8
atpA-atpF_p3 TACTAATCTCGTCGGCTCGA GCTTGACCGACGAGTTACAT 50.0 50.0 57.8 58.0 166-178 bp 1.0 84.4
atpA-atpF_p4 CCGTCGCCTACTTGAAGTAC AGCCTTACAGGGAGCTCTAG 55.0 55.0 58.1 57.9 283-295 bp 1.0 83.9
atpA-atpF_p5 GTCGCCTACTTGAAGTACGG AGCCTTACAGGGAGCTCTAG 55.0 55.0 58.1 57.9 281-293 bp 1.0 83.9