Hotspot

trnG-GCC-trnR-UCU

LSC · 10243-11198 · 956 bp

Back to Mallotus

Pi 0.216

MarkerSeek score 82.20

Primer available yes

Label trnG-GCC-trnR-UCU

Feature Metadata

label_name: trnG-GCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 956
variable_sites: 250
pi: 0.216
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	CGTTTCAAGAGTAGGGTCCG	50.0	55.0	58.0	58.0	1548-1711 bp	1.0	91.7
trnG-GCC-trnR-UCU_p2	AGCCTTCCAAGCTAACGATG	GCGAGTTGCTCAAACAATCC	50.0	50.0	58.0	58.0	1384-1547 bp	1.0	91.5
trnG-GCC-trnR-UCU_p3	CCTAGCCTTCCAAGCTAACG	CGTTTCAAGAGTAGGGTCCG	55.0	55.0	58.1	58.0	1551-1714 bp	1.0	91.5
trnG-GCC-trnR-UCU_p4	CCTAGCCTTCCAAGCTAACG	GCGAGTTGCTCAAACAATCC	55.0	50.0	58.1	58.0	1387-1550 bp	1.0	91.3
trnG-GCC-trnR-UCU_p5	TTTCGCCCACGTCTTATAGC	CGTTTCAAGAGTAGGGTCCG	50.0	55.0	58.1	58.0	1676-1839 bp	0.666667	77.2