Hotspot

trnK-UUU-rps16

LSC · 4724-5270 · 547 bp

Back to Mallotus

Pi 0.0652

MarkerSeek score 86.70

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 547
variable_sites: 51
pi: 0.0652
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 86.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCGCACTTAAAAGCCGAGTA	GGGGGTTACAGAACTTCTTCTC	50.0	50.0	58.3	58.1	656-1187 bp	1.0	68.1
trnK-UUU-rps16_p2	CCGCACTTAAAAGCCGAGTA	GGGGGTTACAGAACTTCTTCT	50.0	47.6	58.3	56.9	656-1187 bp	1.0	68.0
trnK-UUU-rps16_p3	CGCACTTAAAAGCCGAGTAC	GGGGGTTACAGAACTTCTTCTC	50.0	50.0	57.2	58.1	655-1186 bp	1.0	64.1
trnK-UUU-rps16_p4	CGCACTTAAAAGCCGAGTAC	GGGGGTTACAGAACTTCTTCT	50.0	47.6	57.2	56.9	655-1186 bp	1.0	64.0
trnK-UUU-rps16_p5	CCGCACTTAAAAGCCGAGTA	ACTATCCCCCTTCTCTTGTTCT	50.0	45.5	58.3	57.9	633 bp	0.333333	34.4