Hotspot

ycf1

SSC · 124002-129386 · 5385 bp

Back to Malcolmia

Pi 0.00111

MarkerSeek score 53.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5385
variable_sites: 6
pi: 0.00111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GCGACTTTTAGGAAAATTGTTGC	GGGGTTCAACTATCCGAAACA	39.1	47.6	57.7	57.9	347 bp	1.0	46.0
ycf1_p2	GCGACTTTTAGGAAAATTGTTGC	TGGGGTTCAACTATCCGAAAC	39.1	47.6	57.7	57.9	348 bp	1.0	46.0
ycf1_p3	ACCAGAAAAGACTTGGTTTTGA	GGGGTTCAACTATCCGAAACA	36.4	47.6	56.3	57.9	302 bp	1.0	42.5
ycf1_p4	ACCAGAAAAGACTTGGTTTTGA	TGGGGTTCAACTATCCGAAAC	36.4	47.6	56.3	57.9	303 bp	1.0	42.5
ycf1_p5	GCGACTTTTAGGAAAATTGTTGC	TGGGGTTCAACTATCCGAAA	39.1	45.0	57.7	56.4	348 bp	1.0	42.0