Hotspot

rpoC2

LSC · 15782-19921 · 4140 bp

Back to Malcolmia

Pi 0.000725

MarkerSeek score 54.30

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4140
variable_sites: 3
pi: 0.000725
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	ACAACGTTACTGTATGGATCGA	AGAATGGGTCCATTTAATCATGT	40.9	34.8	57.3	55.9	278 bp	1.0	40.3
rpoC2_p2	ACGTTACTGTATGGATCGAGA	AGAATGGGTCCATTTAATCATGT	42.9	34.8	56.0	55.9	275 bp	1.0	40.3
rpoC2_p3	AACAACGTTACTGTATGGATCGA	AGAATGGGTCCATTTAATCATGT	39.1	34.8	57.9	55.9	279 bp	1.0	40.3
rpoC2_p4	CAACGTTACTGTATGGATCGAGA	AGAATGGGTCCATTTAATCATGT	43.5	34.8	58.1	55.9	277 bp	1.0	40.3
rpoC2_p5	ACAACGTTACTGTATGGATCG	AGAATGGGTCCATTTAATCATGT	42.9	34.8	55.8	55.9	278 bp	1.0	40.3