Hotspot

clpP

LSC · 69700-71749 · 2050 bp

Back to Malcolmia

Pi 0.000976

MarkerSeek score 57.60

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2050
variable_sites: 2
pi: 0.000976
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	GATTTGCATGAAATCGCTCCT	ACCGAAACCCCAATCTTACG	42.9	50.0	57.3	57.9	2146 bp	1.0	65.6
clpP_p2	GATTTGCATGAAATCGCTCCT	CCGAAACCCCAATCTTACGT	42.9	50.0	57.3	57.9	2145 bp	1.0	65.6
clpP_p3	ATTTGCATGAAATCGCTCCT	ACCGAAACCCCAATCTTACG	40.0	50.0	56.1	57.9	2145 bp	1.0	63.9
clpP_p4	ATTTGCATGAAATCGCTCCT	CCGAAACCCCAATCTTACGT	40.0	50.0	56.1	57.9	2144 bp	1.0	63.9
clpP_p5	GATTTGCATGAAATCGCTCC	CCGAAACCCCAATCTTACGT	45.0	50.0	55.8	57.9	2145 bp	1.0	61.5