Hotspot

cemA

LSC · 60533-61222 · 690 bp

Back to Malcolmia

Pi 0.00145
MarkerSeek score 59.60
Primer available yes
Label cemA

Feature Metadata

label_name
cemA
feature_type
gene
region
LSC
length_bp
690
variable_sites
1
pi
0.00145
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
cemA_p1 TGGATTCATAGGCTCACTACA ACAAGGAATCCCTCGTGGTA 42.9 50.0 55.9 58.1 912 bp 1.0 54.6
cemA_p2 TGGATTCATAGGCTCACTACA TACAAGGAATCCCTCGTGGT 42.9 50.0 55.9 58.1 913 bp 1.0 54.6
cemA_p3 AGGCTCACTACATTCTATTCGA ACAAGGAATCCCTCGTGGTA 40.9 50.0 56.5 58.1 903 bp 1.0 51.3
cemA_p4 AGGCTCACTACATTCTATTCGA TACAAGGAATCCCTCGTGGT 40.9 50.0 56.5 58.1 904 bp 1.0 51.3
cemA_p5 TGGATTCATAGGCTCACTACAT ACAAGGAATCCCTCGTGGTA 40.9 50.0 56.4 58.1 912 bp 1.0 50.9