Hotspot

trnN-AAC-trnR-CGU

IRa · 129779-130413 · 635 bp

Back to Malcolmia

Pi 0.00157

MarkerSeek score 59.70

Primer available yes

Label trnN-AAC-trnR-CGU

Feature Metadata

label_name: trnN-AAC-trnR-CGU
feature_type: igs
region: IRa
length_bp: 635
variable_sites: 1
pi: 0.00157
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-AAC-trnR-CGU_p1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	776 bp	1.0	78.4
trnN-AAC-trnR-CGU_p2	CCGTTGTTCCTCAGTAGCTC	GAACAGATCCAGTGGAGACG	55.0	55.0	58.0	57.7	813 bp	1.0	77.8
trnN-AAC-trnR-CGU_p3	TGGGTTGAGAACGGGAATTG	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.1	57.8	817 bp	1.0	77.6
trnN-AAC-trnR-CGU_p4	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	727 bp	1.0	77.1
trnN-AAC-trnR-CGU_p5	TGGGTTGAGAACGGGAATTG	GAACAGATCCAGTGGAGACG	50.0	55.0	58.1	57.7	854 bp	1.0	77.0