Hotspot

trnR-CGU-trnN-AAC

IRb · 108487-109121 · 635 bp

Back to Malcolmia

Pi 0.00157

MarkerSeek score 59.70

Primer available yes

Label trnR-CGU-trnN-AAC

Feature Metadata

label_name: trnR-CGU-trnN-AAC
feature_type: igs
region: IRb
length_bp: 635
variable_sites: 1
pi: 0.00157
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-CGU-trnN-AAC_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	776 bp	1.0	78.4
trnR-CGU-trnN-AAC_p2	GAACAGATCCAGTGGAGACG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.7	58.0	813 bp	1.0	77.8
trnR-CGU-trnN-AAC_p3	CAGAGGATTAGAGCACGTGG	TGGGTTGAGAACGGGAATTG	55.0	50.0	57.8	58.1	817 bp	1.0	77.6
trnR-CGU-trnN-AAC_p4	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	727 bp	1.0	77.1
trnR-CGU-trnN-AAC_p5	GAACAGATCCAGTGGAGACG	TGGGTTGAGAACGGGAATTG	55.0	50.0	57.7	58.1	854 bp	1.0	77.0