Hotspot

psaJ-rpl33

LSC · 66818-67265 · 448 bp

Back to Malcolmia

Pi 0.00495

MarkerSeek score 61.90

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 448
variable_sites: 2
pi: 0.00495
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTCGGTTCGTTAGCAGGTTT	TCTGGAAATTCCCGCTGATT	45.0	45.0	57.8	57.2	581-625 bp	1.0	75.9
psaJ-rpl33_p2	TTCGGTTCGTTAGCAGGTTT	TGGAAATTCCCGCTGATTCT	45.0	45.0	57.8	57.2	579-623 bp	1.0	75.9
psaJ-rpl33_p3	ATGGTTCGGTTCGTTAGCAG	TCTGGAAATTCCCGCTGATT	50.0	45.0	58.3	57.2	585-629 bp	1.0	75.6
psaJ-rpl33_p4	ATGGTTCGGTTCGTTAGCAG	TGGAAATTCCCGCTGATTCT	50.0	45.0	58.3	57.2	583-627 bp	1.0	75.6
psaJ-rpl33_p5	TTCGGTTCGTTAGCAGGTTT	AACATCTTTACCCTTGGCCA	45.0	45.0	57.8	57.0	506-550 bp	1.0	74.4