Hotspot

ndhC-trnV-GUA

LSC · 50202-51047 · 846 bp

Back to Malcolmia

Pi 0.00236
MarkerSeek score 60.10
Primer available yes
Label ndhC-trnV-GUA

Feature Metadata

label_name
ndhC-trnV-GUA
feature_type
igs
region
LSC
length_bp
846
variable_sites
2
pi
0.00236
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-GUA_p1 TGCCAAAACAGGAATAGCACT GTTCGAGTCCGTATAGCCCT 42.9 55.0 58.1 58.7 939-940 bp 1.0 65.7
ndhC-trnV-GUA_p2 TGCCAAAACAGGAATAGCACT TTCGAGTCCGTATAGCCCTA 42.9 50.0 58.1 57.1 938-939 bp 1.0 63.7
ndhC-trnV-GUA_p3 TGCCAAAACAGGAATAGCAC GTTCGAGTCCGTATAGCCCT 45.0 55.0 56.6 58.7 939-940 bp 1.0 63.5
ndhC-trnV-GUA_p4 TGCCAAAACAGGAATAGCACT TCGAACTCTTTTACCGAGCAG 42.9 47.6 58.1 58.1 967-968 bp 1.0 62.7
ndhC-trnV-GUA_p5 TGCCAAAACAGGAATAGCACT TCGAACTCTTTTACCGAGCA 42.9 45.0 58.1 56.9 967-968 bp 1.0 62.4