Hotspot

ndhJ-psbG

LSC · 48978-49079 · 102 bp

Back to Malcolmia

Pi 0.0196

MarkerSeek score 63.80

Primer available yes

Label ndhJ-psbG

Feature Metadata

label_name: ndhJ-psbG
feature_type: igs
region: LSC
length_bp: 102
variable_sites: 2
pi: 0.0196
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-psbG_p1	CCTTGGTAATCGAAGCCCAA	AGTCCAGTATCTTCCCACGA	50.0	50.0	57.9	57.5	209 bp	1.0	75.6
ndhJ-psbG_p2	GCCCAACGATCTATGAACCA	AGTCCAGTATCTTCCCACGA	50.0	50.0	57.7	57.5	195 bp	1.0	74.0
ndhJ-psbG_p3	TTCCTTGGTAATCGAAGCCC	AGTCCAGTATCTTCCCACGA	50.0	50.0	57.6	57.5	211 bp	1.0	73.2
ndhJ-psbG_p4	CGAAGCCCAACGATCTATGA	AGTCCAGTATCTTCCCACGA	50.0	50.0	57.5	57.5	199 bp	1.0	72.5
ndhJ-psbG_p5	TCGAAGCCCAACGATCTATG	AGTCCAGTATCTTCCCACGA	50.0	50.0	57.5	57.5	200 bp	1.0	72.5