Hotspot

psaA-ycf3

LSC · 41525-42236 · 712 bp

Back to Malcolmia

Pi 0.00141

MarkerSeek score 59.80

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 712
variable_sites: 1
pi: 0.00141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	ATTGGTTGACGATCACGAGG	50.0	50.0	57.9	58.0	822-824 bp	1.0	79.3
psaA-ycf3_p2	AGAAATGACCGGGTTTAGCC	ATTGGTTGACGATCACGAGG	50.0	50.0	57.9	58.0	839-841 bp	1.0	79.2
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	AGCACAGAATTGGTTGACGA	50.0	45.0	57.9	57.7	830-832 bp	1.0	77.3
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	AGCCGCTGAGTATTGGAAAC	50.0	50.0	57.9	58.3	884-886 bp	1.0	77.3
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	CAAGCCGCTGAGTATTGGAA	50.0	50.0	57.9	58.3	886-888 bp	1.0	77.3